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调节性T细胞(Treg)检测试剂盒

放大字体  缩小字体 发布日期:2014-06-04  作者:欣博盛  浏览次数:21
公司介绍:欣博盛生物科技有限公司

 


天然CD4+ CD25+调节性T细胞( regulatory Tcell,Treg )为一类具有免疫抑制作用的T细胞,约占外周CD4+ T细胞的5~10%。该类细胞以"主动"的方式参与免疫应答/免疫耐受的调控, 不仅参与自身免疫耐受调节,而且在肿瘤免疫和移植免疫中也具有重要的作用。Foxp3( forkhead box P3,Scurfin)是目前公认的Treg细胞最特征性的标志,其表达及功能与Treg细胞密切相关。Foxp3主要表达于胸腺、脾脏和淋巴结等淋巴器官和组织,主要通过流式细胞术来检测,美国eBioscience公司是最早拥有Foxp3流式检测抗体的公司之一,公司通过不断改进和优化,建立了一套完美的针对Foxp3流式检测特殊的试剂和方案。

 
 供货商
 产品编号
 产品名称
 规格
 成分
 eBioscience
 88-4994-40
 Human/Non-Human Primate Regulatory T Cell Staining Kit #2 Kit
 Kit (25 tests)
 FITC anti-human CD4 (OKT4): 25 tests.
 PE anti-human CD25 (BC96): 25 tests.
 APC anti-human Foxp3 (PCH101): 25 tests.
 APC Rat IgG2a isotype control: 25 tests.
 Normal Rat Serum: 100 µl.
 eBioscience Flow Cytometry Staining Buffer: 200 ml.
 eBioscience Fixation/Permeabilization Concentrate: 30ml.
 eBioscience Fixation/Permeabilization Diluent: 100 ml.  
 eBioscience Permeabilization Buffer (10X): 100 ml.
 未提供:Isotype controls for anti--
 CD4(mouse IgG1, cat. 11‐4714) and anti‐
 CD25 (mouse IgG1, cat. 12‐4714)
 eBioscience
 88-4999-40
 Human/Non-Human Primate Regulatory T Cell Staining Kit #1Kit
 Kit (25 tests)
 FITC anti-human CD4 (OKT4): 25 tests.
 APC anti-human CD25 (BC96): 25 tests.
 PE anti-human Foxp3 (PCH101): 25 tests.
 PE Rat IgG2a isotype control: 25 tests.
 Normal Rat Serum: 100 µl.
 eBioscience Flow Cytometry Staining Buffer: 200 ml.
 eBioscience Fixation/Permeabilization Concentrate: 30 ml.
 eBioscience Fixation/Permeabilization Diluent: 100 ml.
 eBioscience Permeabilization Buffer (10X): 100 ml.
 未提供:Isotype controls for anti‐
 CD4 (mouse IgG1, cat. 11‐4714) and anti‐
 CD25 (mouse IgG1, cat. 17‐4714)
 eBioscience
 88-8111-40
 Mouse Regulatory T
 Cell Staining Kit #1 Kit
 Kit (50 tests)
 FITC anti-mouse CD4 (RM4-5): 50 µg. Use at 0.125 µg/test.
 APC anti-mouse CD25 (PC61.5): 50 µg. Use at 0.06 µg/test.
 PE anti-mouse/rat Foxp3 (FJK-16s): 25 µg. Use at 0.5 µg/test.
 PE Rat IgG2a isotype control: 50 µg. Use at 0.5 µg/test.
 Affinity Purified anti-mouse CD16/32 (Fc Block): 50 µg. Use at 1-5 µg/test.
 eBioscience Flow Cytometry Staining Buffer: 200 ml.
 eBioscience Fixation/Permeabilization Concentrate: 30 ml.
 eBioscience Fixation/Permeabilization Diluent: 100 ml.
 eBioscience Permeabilization Buffer (10X): 100 ml.
 未提供:Isotype controls for anti-
 CD4 (rat IgG2a, cat. 11-4321) and anti-
 CD25 (rat IgG1, cat. 17-4301)
 eBioscience
 88-8115-40
 Mouse Regulatory T
 Cell Staining Kit #3 Kit
 Kit
 FITC anti-mouse CD4 (RM4-5): 50 µg. Use at 0.125 µg/test.
 PE anti-mouse CD25 (PC61.5): 50 µg. Use at 0.06 µg/test.
 PE-Cy5 anti-mouse/rat Foxp3 (FJK-16s): 25 µg. Use at 0.5 µg/test.
 PE-Cy5 Rat IgG2a isotype control: 25 µg. Use at 0.5 µg/test.
 Affinity Purified anti-mouse CD16/32 (Fc Block): 50 µg. Use at 1-5 µg/test
 eBioscience Flow Cytometry Staining Buffer: 200 ml
 eBioscience Fixation/Permeabilization Concentrate: 30 ml. eBioscience  
 Fixation/Permeabilization Diluent
: 100 ml.
 eBioscience Permeabilization Buffer (10X): 100 ml.
 未提供:Isotype controls for anti-
 CD4 (rat IgG2a, cat. 11-4321) and anti-
 CD25 (rat IgG1, cat. 12-4301)
 eBioscience
 88-8118-40
 Mouse Regulatory T
 Cell Staining Kit #2 Kit
 Kit
 FITC anti-mouse CD4 (RM4-5): 50 µg. Use at 0.125 µg/test.
 PE anti-mouse CD25 (PC61.5): 50 µg. Use at 0.06 µg/test.
 APC anti-mouse/rat Foxp3 (FJK-16s): 25 µg. Use at 0.5 µg/test.
 APC Rat IgG2a isotype control: 50 µg. Use at 0.5 µg/test.
 Affinity Purified anti-mouse CD16/32 (Fc Block): 50 µg Use at 2-5 µg/test.
 Flow Cytometry Staining Buffer: 200 ml
 Fixation/Permeabilization Concentrate: 30 ml.Fixation/Permeabilization Diluent: 100 ml. Store at 2-8°C.
 Permeabilization Buffer (10X): 100 ml.
 未提供:Isotype controls for anti-
 CD4 (rat IgG2a, Cat. No. 11-4321) and anti-
 CD25 (rat IgG1, Cat. No. 12-4301)
 eBioscience
 88-8995-40
 Human Regulatory T
 Cell Staining Kit #3 Kit
 Kit
 Cocktail of FITC anti-human CD4 (RPA-T4) and PE anti-human CD25 (BC96): 25 tests.
 PE-Cy5 anti-human Foxp3 (PCH101): 25 tests.
 PE-Cy5 Rat IgG2a isotype control: 25 µg.
 Normal Rat Serum: 100 µl.
 eBioscience Flow Cytometry Staining Buffer: 200 ml.
 eBioscience Fixation/Permeabilization   Concentrate: 30 ml.
 eBioscience Fixation/Permeabilization Diluent: 100 ml. 
 eBioscience Permeabilization Buffer (10X): 100 ml.
 未提供:
 12-4714  Mouse IgG1 K Isotype Control PE       
 11-4714  Mouse IgG1 K Isotype Control FITC
 eBioscience
 88-8998-40
 Human Regulatory T
 Cell Staining Kit #2Kit
 Kit
 Cocktail of FITC anti-human CD4 (RPA-T4) and PE anti-human CD25 (BC96): 25 tests.
 APC anti-human Foxp3 (PCH101): 25 tests.
 APC Rat IgG2a isotype control: 25 tests.
 Normal Rat Serum: 100 µl.
 eBioscience Flow Cytometry Staining Buffer: 200 ml.
 eBioscience Fixation/Permeabilization Concentrate: 30 ml. 
 eBioscience Fixation/Permeabilization Diluent: 100 ml.
 eBioscience Permeabilization Buffer (10X): 100 ml.
 未提供:
 12-4714  Mouse IgG1 K Isotype Control PE     
 11-4714  Mouse IgG1 K Isotype Control FITC
 eBioscience
 88-8999-40
 Human Regulatory T
 Cell Staining Kit Kit
 Kit
 Cocktail of FITC anti-human CD4 (RPA-T4) and APC anti-human CD25 (BC96): 25 tests.
 PE anti-human Foxp3 (PCH101): 25 tests.
 PE Rat IgG2a isotype control: 25 tests.
 Normal Rat Serum: 100 µl.
 eBioscience Flow Cytometry Staining Buffer: 200 ml.
 eBioscience Fixation/Permeabilization Concentrate: 30 ml.
 eBioscience Fixation/Permeabilization Diluent: 100 ml.
 eBioscience Permeabilization Buffer (10X): 100 ml.
 未提供:
 11-4714  Mouse IgG1 K Isotype Control FITC 
 17-4714  Mouse IgG1 K Isotype Control APC
 eBioscience
 88-8996-40
 Human Regulatory T
 Cell Whole Blood
 Staining Kit
 Kit
 eBioscience 1X RBC Lysis Buffer: 200 ml
 eBioscience Flow Cytometry Staining Buffer: 600 ml
 eBioscience Fixation/Permeabilization Concentrate: 30 ml
 eBioscience Fixation/Permeabilization Diluent: 100 ml
 PE anti-human Foxp3 (PCH101): 25 tests
 未提供:
 CD4 抗体和CD25抗体及其同型对照
 12-4321 Rat IgG2a K Isotype Control PE (eBR2a)

 

 
关键词: Treg Treg检测
 
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