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3.5KD到240KD的广谱蛋白预染marker,8折促销!
促销时间:2013-11-01至2013-12-30        浏览次数:48        

巴傲得生物,Bioworld品牌,货号BD0027, 3.5KD到240KD的广谱蛋白预染marker,大家感觉怎么样?价格也不贵哦!蛋白marker(3.5kd,8kd,15kd,18kd,24kd,31kd,42kd,57kd,72kd,93kd,125kd,165kd,240kd)


 

Size: 500 μl

• 3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively.

• 1.5~2.5 μl per well for general Western transferring.

• Apply more for thicker (> 1.5 mm) or larger gel.

Description

The BLUelf Prestained Protein Ladder is a three-color protein standard with 13 pre-stained proteins covering a wide

range molecular weights from 3.5 to 245 kDa. Proteins are covalently coupled with a blue chromophore except for

two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE

(Tris-glycine buffer). The BLUelf Prestained Protein Ladder is designed for monitoring protein separation during

SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or

nitrocellulose) and for approximating the size of proteins. The ladder is supplied in gel loading buffer and is ready

to use. Do not heat, dilute, add reducing agent before loading.

Contents

Approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate, pH 7.5 at 25°C), 2 % SDS, 10 mM

Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol).

 

 


 
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