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pull-down or G-LISA试剂盒65折大促销
促销时间:2014-06-01至2014-07-10        浏览次数:62        

 小G蛋白研究利器-- pull-down or G-LISA试剂盒65折大促销
上海优宁维生物科技有限公司

活动时间:2014年6月1日-7月10日

 

近几年,测定细胞或组织中的小G蛋白的活性已经成为信号转导研究者广泛应用的技优LOGO新2术。小G蛋白中的Rho家族包含至少有20个成员,其中最有特性研究做多的是Rho、Rac、 Rrf 、Cdc42、Ras、Ral 。来自美国的Cytoskeleton公司为小G蛋白活性检测提供了两种方法:pull-down(Affinity bead followed by Western blot format)和G-Lisa(Elisa based format).
 

QQ图片20140523134329

Choose Your Format 

  G-LISA® Pull-down
Total protein per assay 10-50 μg 500-2000 μg
Assay time <3 h 10-12 h (2 days)
Lysate clarification needed*  No Yes
Sample handling 96 Samples 10 Samples
Quantitative data** Yes Semi


QQ图片20140523134548


Pull-down Activation Assays 

Pull-down assays utilize affinity beads linked to an effector protein that selectively binds active GTPase followed by quantitation with Western blotting.

 

Pull-down Activation Assays Cat. # Amount
Combo RhoA/Rac1/Cdc42 Activation Assay Biochem Kit BK030 3 x 10 assays
Arf1 Activation Assay Biochem Kit™ BK032-S 20 assays
Arf6 Activation Assay Biochem Kit™ BK033-S 20 assays
Cdc42 Activation Assay Biochem Kit™ BK034-S 20 assays
BK034 50 assays
Rac1 Activation Assay Biochem Kit™ BK035-S 20 assays
BK035 50 assays
RalA Activation Assay Biochem Kit™  BK040 50 assays
Ras Activation Assay Biochem Kit™ BK008-S 20 assays
  BK008 50 assays
RhoA Activation Assay Biochem Kit™ BK036-S 20 assays
BK036 50 assays

QQ图片20140523134555


G-LISA® Activation Assays

G-LISAs use a 96-well plate coated with effector protein that selectively binds the active GTPase followed by quantitation with ELISA techniques.

 

G-LISA Activation Assays Cat. # Amount
G-LISA RhoA Activation Assay Biochem Kit (luminescence format)

BK121

96 assays
G-LISA RhoA Activation Assay Biochem Kit (colorimetric format) BK124 96 assays
G-LISA Rac1,2,3 Activation Assay Biochem Kit (colorimetric format) BK125 96 assays
G-LISA Rac1 Activation Assay Biochem Kit (luminescence format) BK126 96 assays
G-LISA Cdc42 Activation Assay Biochem Kit (colorimetric format) BK127 96 assays
G-LISA Rac1 Activation Assay Biochem Kit (Colorimetric Based) BK128 96 assays
G-LISA RalA Activation Assay Biochem Kit (Colorimetric Based) BK129 96 assays
G-LISA Ras Activation Assay Biochem Kit (Colorimetric Based) BK131 96 assays
G-LISA Arf1 Activation Assay Biochem Kit (Absorbance Based) BK132 96 assays
G-LISA Arf6 Activation Assay Biochem Kit (Absorbance Based) BK133 96 assays

 

上海优宁维生物科技有限公司(www.univ-bio.com)

 

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