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南京三辰生物狂犬抗原(Rabies virus-Ag)促销
促销时间:2015-03-31至2015-06-30        浏览次数:36        

  

狂犬抗原(Rabies virus-Ag)使用说明书                             

                         

一、 技术参数:(0.1微克可检测1人份)                                                                           

含量:0.5mg/ml                                                                                                         

纯度:≥95%                                                                                                              

分子量:56KD                                                                                                               

存放液:PH7.9   PBS--6M尿素--40%甘油                                                                      

二、 建议使用方法:(1:500—1:1000稀释使用)                                                                 

包被:PH9.6  0.05M碳酸盐缓冲液                                                                                

      37℃放置3h后4℃过夜                                                                                        

封闭:20%小牛血清                                                                                                    

      37℃放置3h                                                                                                      

三、 ELISA方法(1: 500包被)                                                                                           

在酶标包被板中每孔加入100微升样品稀释液;                                                             

在阴、阳性对照孔中加入100微升对应阴性对照,阳性对照;                                          

在待测样品孔中每孔加入5微升待测样品,置37℃,30min甩出,洗板5次(洗液建议使用PBST);                                                                                                  

每孔加入酶结合物100微升,30min甩出,洗板5次;                                                       

每孔加入底物液50微升,显色剂50微升,10min,再每孔加入终止液50微升;观察结果。   

     FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES

                                                                                                                                                        

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