Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral respo
nses upon binding Ca2+ have enabled researchers to investigate changes in intracellular free Ca2+ co
ncentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Rhod-2 is most commo
nly used among the red fluorescent calcium indicators. However, Rhod-2 AM is o
nly moderately fluorescent in live cells upon esterase hydrolysis, and has very small cellular calcium responses. Quest Rhod-4™ has been developed to improve Rhod-2 cell loading and calcium respo
nse while maintaining the spectral wavelength of Rhod-2. In CHO and HEK cells Quest Rhod-4™ AM has cellular calcium respo
nse that is 10 times more sensitive than Rhod-2 AM. AAT Bioquest offers versatile packing sizes of Quest Rhod-4 to meet your special needs, e.g., 1 mg; 10x50 µg; 20x50 µg; HTS packages with no additio
nal packaging charge.