ndria are major producers of cellular superoxide. The production of low to moderate levels of superoxide is critical for the proper regulation of many essential cellular processes including gene ex
pression, signal transduction, and muscle adaptation to endurance exercise training. Unco
ndrial superoxide production can trigger cellular oxidative damage that co
ntributes to the pathogenesis of a wide variety of disorders including cancer, cardiovascular diseases, neurodegenerative diseases and aging. Therefore, the detection of intracellular mitocho
ndrial superoxide is of central im
portance to understanding proper cellular redox regulation and the impact of its dysregulation on various pathologies. Cell Meter™ Fluorimetric Mitocho
ndrial Superoxide Activity Assay Kit uses our unique superoxide indicator to quantify superoxide level in live cells. MitoROS™ 580 is live-cell permeant and can rapidly and selectively target superoxide in mitochondria. It generates red fluorescence when it reacts with superoxide, and can be easily read at Ex/Em = 540/590 nm. The Cell Meter™ Fluorimetric Intracellular Superoxide Detection Kit provides a sensitive, one-step fluorimetric assay to detect mitocho
ndrial superoxide in live cells with one hour incubation. This kit is optimized for flow cytometry applications.