Autophagy is one of the major pathways for degradation of intracellular macromolecules in animal cells. The process of autophagy involves the sequestration of cytoplasmic materials and intracellular organelles in a membrane-bounded vacuole called autophagosome, the fusion of the autophagosome with lysosomes, and the subsequent degradation of sequestered materials. Cell Explorer� autophagy fluorescence imaging kit employs Autophagy Super Blue� as a specific autophagosome marker to analyze the activity of autophagy. The assay is optimized for direct detection of autophagy in both detached and attached cells. The kit provides all the essential compo
nents for the assay protocol. The Cell Explorer� autophagy fluorescence imaging kit is optimized for fluorescence microscope, it is also suitable for flow cytometer. Autophagy Super Blue� has fluorescence excitation and emission at Ex/Em = 333/518 nm. It gives much higher selectivity than other commercially available autophagy probes.