Fluoro-Jade® B is a polyanio
nic fluorescein derivative which sensitively and specifically binds to degenerating neurons. It has a green fluorescence with excitation peak at 480 nm and emission peak at 525 nm. The filter used for visualizing Fluoro-Jade B is the FITC filter. Fluoro-Jade B can be used on most tissue section types and thicknesses including frozen, vibratomed cryostat or paraffin-embedded sections from 3-50 um. Fluoro-Jade B is faster and more reliable than older methods (e.g. suppressed silver) for the unequivocal qualitative detection and quantitative measurement of both gross and fine scale neuro
nal degeneration. Fluoro-Jade B will not distinguish between apoptotic and necrotic neurons. Astrocytic labeling is occasio
nally observed with Fluoro-Jade B, especially in chro
nic studies. Fluoro-Jade® is a registered trademark of Histo-Chem, Inc.