Fluoro-Jade® C, like its predecessors, Fluoro-Jade and Fluoro-Jade B stain all degenerating neurons, regardless of specific insult or mechanism of cell death. Therefore, the patterns of neuro
nal degeneration seen following exposure to either the glutamate agonist, kainic acid, or the inhibitor of mitocho
ndrial respiration, 3-NPA, were the same for all of the Fluoro-Jade dyes. However, there was a qualitative difference in the staining characteristics of the three fluorochromes. Specifically, Fluoro-Jade C exhibited the greatest signal to background ratio, as well as the highest resolution. This translates to a stain of maximal co
ntrast and affinity for degenerating neurons. This makes it ideal for localizing not o
nly degenerating nerve cell bodies, but also distal dendrites, axons and terminals. The dye is highly resistant to fading and is compatible with virtually all histological processing and staining protocols. Triple labeling can be accomplished by staining degenerating neurons with Fluoro-Jade C, cell nuclei with DAPI and activated astrocytes with GFAP immunofluorescence. Fluoro-Jade® is a registered trademark of Histo-Chem, Inc.