Texas Red� DHPE is a phospholipid that is labeled on the head group with the bright, red-fluorescent Texas Red� dye (excitation/emission maxima ~595/615 nm). Rhodamine-labeled phospholipids have been used as tracers for membrane traffic during endocytosis and for lipid processing in hepatocytes. Texas Red DHPE is principally employed as an energy transfer acceptor from NBD, BODIPY and fluorescein lipid probes. The lo
nger emission wavelength of the Texas Red dye provides superior separation of the do
nor and acceptor emission signals in reso
nance energy transfer microscopy. This technique has enabled visualization of ATP-dependent fusion of liposomes with the Golgi apparatus. Membrane flux during hemagglutinin-mediated cell朿ell fusion has been visualized using Texas Red DHPE and the lipophilic carbocyanine DiI as membrane labels.