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赢润生物 YRGene pYES2/CT/α-Factor 载体 质粒

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品牌: YRGene
型号: VJI0571
规格: 一管质粒,5ug。附送一管甘油菌,500ul。
单价: 900.00元/
发货期限: 自买家付款之日起 3 天内发货
所在地: 湖南 长沙市
有效期至: 长期有效
浏览次数: 13
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产品详细说明
产品具体信息请参见:
http://www.yrgene.com/goods-123162.html
货号:
VJI0571    
载体名称:pYES2/CT/α-Factor    
出品公司:Invitrogen    
载体用途:酵母表达载体    
价格:900元
规格:一管质粒,5ug。附送一管甘油菌,500ul。    
到货周期:现货
载体用途:酿酒酵母表达载体
诱导方法:半乳糖
原核抗性:Ampicillin(氨苄青霉素)
筛选标记:URA3
启动子:GAL1 promter
标签:C端带V5标签及His标签
5' 测序引物:GAL1-f(5'-AATATACCTCTATACTTTAACGTC-3')
3' 测序引物:CYC1-r(5'-GCGTGAATGTAAGCGTGAC-3')
备注:利用半乳糖诱导蛋白在酿酒酵母中表达。


载体描述:
pPIC9 was used as a template and α-factor gene of Saccharomyces cerevisiae was amplified by PCR. The gene was cloned in the intracellular expression vector pYES2/CT for Saccharomyces cerevisiae and the secreting expression vector named pYES2/CT/α-factor(pYCα) was constructed. The gene of mannase(man) from recombinant vector of pKLAC1-man (pKLman) was cut by restriction enzymes and linked with pYCα. This recombinant vector pYCα-man was used to determine the secretory ability and stability of pYCα. The excellent secretory ability of pYCα was proved by two experiments. One showed that INVSc1/pYCα-man clones formed the clear rings around the clones on the medium contained trypan blue, while INVSc1/pYCα clones had no rings.Further analysis of mannase activity of extracellular supernatant and intracellular extracts showed that both extracellular and intracellular mannase activities of INVSc1/pYCα were not detected,while INVScl/pYCα-man had evident extracellular mannase activities and no intracellular mannase activities. The stability of pYCα was also very good proved by continuous cultivation for about 150h.

以pPIC9为模板,通过PCR扩增获得酿酒酵母的α-交配因子(α-factor),并克隆至酿酒酵母胞内表达载体pYES2/CT中,构建了一种新型酿酒酵母附加型分泌表达载体pYES2/CT/α-factor(pYCα)。

产品说明书及载体序列:
请访问以下页面下载【pYES2/CT/α-Factor产品说明书及载体序列:
http://www.yrgene.com/goods-123162.html

质粒图谱:

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